Journal: PLoS Pathogens
Article Title: Deciphering the molecular mechanisms of mother-to-egg immune protection in the mealworm beetle Tenebrio molitor
doi: 10.1371/journal.ppat.1008935
Figure Lengend Snippet: Antimicrobial activity was measured in 3 days old eggs originated from females that were primed and injected with either PBS (PBS), dsRNA from non-relevant GFP (dsGFP) or dsRNA targeting the five candidate AMPs (dsAMP). Eggs were laid 5 days after priming. A. The zone of inhibition around eggs disposed on A . globiformis -covered agar plates was measured. Eggs that did not exhibit antimicrobial activity (null zone of inhibition) were not considered. B. The percentage of eggs exhibiting antibacterial activity is represented for the same three samples. A star ‘*’ above the bar indicates a significant difference between the dsAMP condition and the PBS and dsGFP samples ( p < 0.05; Fisher’s exact test).
Article Snippet: After centrifugation (3,500 g, 2 min, 4°C), 2 mL of the supernatant was used to measure antibacterial activity on zone of inhibition plates seeded with the bacterium Arthrobacter globiformis , obtained from the Pasteur Institute (CIP105365).
Techniques: Activity Assay, Injection, Inhibition